New cell separation technique for the isolation and analysis of cells from biological mixtures in forensic caseworks

作者：Li Cai xia; Wang Gui qiang; Li Wan shui; Huang Jiang ping; Ji An quan; Hu Lan^*

来源：Croatian Medical Journal, 2011, 52(3): 293-298.

DOI：10.3325/cmj.2011.52.293

Aim To isolate mucosal cells of the perpetrator in a sexual assault case from a complex mixture of his mucosal cells and the victim's skin by micromanipulation prior to genomic analysis.
Methods To capture and analyze mucosal cells we used the micromanipulation with on-chip low volume polymerase chain reaction (LV-PCR). Consensus DNA profiles were generated from 5 replicate experiments.
Results and conclusions We validated the use of micromanipulation with on-chip LV-PCR for genomic analysis of complex biological mixtures in a fatal rape case. The perpetrator's mucosal cells were captured from nipple swabs of the victim, and a single-source DNA profile was generated from cell mixtures. These data suggest that micromanipulation with on-chip LV-PCR is an effective forensic tool for the analysis of specific cells from complex samples.

• 出版日期2011-6
• 单位司法部司法鉴定科学技术研究所; 中国人民公安大学