A hyaluronic acid dispersed carbon nanotube electrode used for a mediatorless NADH sensing and biosensing

作者：Filip Jaroslav; Sefcovicova Jana; Tomcik Peter; Gemeiner Peter; Tkac Jan^*

来源：Talanta, 2011, 84(2): 355-361.

DOI：10.1016/j.talanta.2011.01.004

• Sensor
• Carbon nanotubes
• Hyaluronic acid
• NADH
• FIA

A biocompatible nanocomposite consisting of single-walled carbon nanotubes (CNTs) dispersed in a hyaluronic acid (HA) was investigated as a sensing platform for a mediatorless electrochemical detection of NADH. The device was characterised by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and extensively by electrochemistry. CNT-HA bionanocomposite showed more reversible electrochemistry, higher short-term stability of NADH sensing and higher selectivity of NADH detection compared to frequently used CNT-CHI (chitosan) modified GCE. Finally the performance of the sensor modified by CNT-HA was tested in a batch and flow injection analysis (FIA) mode of operation with basic characteristics revealed. The NADH sensor exhibits a good long-term operational stability (95% of the original sensitivity after 22h of continuous operation). Subsequently a D-sorbitol biosensor based on such a nanoscale built interface was prepared and characterised with a D-sorbitol dehydrogenase used as a biocatalyst.

• 出版日期2011-4-15