Gene expression profiles of adipose tissue of high-fat diet-induced obese rats by cDNA microarrays

作者：Qiu Jie; Cheng Rui; Zhou Xiao yu; Zhu Jin gai; Zhu Chun; Qin Da ni; Kou Chun zhao; Guo Xi rong^*

来源：Molecular Biology Reports, 2010, 37(8): 3691-3695.

DOI：10.1007/s11033-010-0021-6

• High-fat diet
• Adipose tissue
• cDNA microarrays
• Gene expression
• Obesity

To better understand the molecular basis of dietary obesity, we examined adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity using cDNA microarrays. Male Sprague-Dawley rats were fed either the HFD or the normal diet. Seven weeks later, the weights of obese models (362.92 +/- A 39.65 g) were significantly higher than those of normal control rats (315.22 +/- A 42.30 g, P < 0.01) and the wet weights of adipose tissue of rats fed with HFD (9.29 +/- A 5.14 g) were significantly higher than those of normal control rats (4.09 +/- A 2.69 g, P < 0.01), which confirmed the successful preparation of obese models. cDNA microarrays containing 9 216 genes/Ests were used to investigate gene expression of adipose tissue. Autoradiographic analysis showed that 532, 154, and 22 genes were differently expressed over 2-, 3-, and 5-fold, respectively. The analysis of gene expression profiles indicated that 276 genes were up-regulated and 432 genes were down-regulated in response to HFD-induced obesity. Different clusters of genes associated with lipid metabolism, extracellular matrix, signal transduction, cytoskeleton, cell apoptosis, etc., such as VLCS-H2, DGAT, ACADVL, PHYH, SCD, ACACA, ACS, MMP-2, MMP-15, CD38, CAMK2D, CACNA1F, CAPZA2, TMOD3, ARPC2, KNS2, TPM1, MAPK8, GADD45B, DAXX, TOK-1, PRKACA, STAT6, were concerned.

• 出版日期2010-12
• 单位南京医科大学