Draft genome sequence of Enterobacter cloacae ST520 harbouring bla(KPC-2), bla(CTX-M-15) and bla(OXA-17) isolated from coastal waters of the South Atlantic Ocean

作者：Sellera Fabio P; Fernandes Miriam R; Moura Quezia; Souza Tiago A; Cerdeira Louise; Lincopan Nilton^*

来源：Journal of Global Antimicrobial Resistance, 2017, 10: 279-280.

DOI：10.1016/j.jgar.2017.07.017

• Enterobacter cloacae
• Seawater
• bla(KPC-2)
• bla(CTX-M-15)
• Whole-genome sequencing

Objectives: Aquatic environments have contributed to the dissemination of multidrug-resistant (MDR) bacteria, representing a risk for humans and animals. The aim of this study was to report the first draft genome sequence of a MDR Enterobacter cloacae strain recovered from seawater in a public beach in Brazil.
Methods: The genome was sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using SPAdes 3.10.1 and the whole genome sequence was analysed using bioinformatics tools from the Center of Genomic Epidemiology.
Results: This draft genome resulted in 5 228 857 bp with 5331 protein-coding sequences, revealing the presence of bla(KPC-2), bla(CTX-M-15) and bla(OXA-17) genes, responsible for resistance to all beta-lactam antibiotics. In addition, the strain was assigned to sequenced type 520 (ST520).
Conclusion: These data provide useful information for comparative genomic analysis regarding the dissemination of antibiotic resistance genes.

• 出版日期2017-9