Alkaloids from Peumus boldus and their Acetylcholinesterase, Butyrylcholinesterase and Prolyl Oligopeptidase Inhibition Activity

作者：Host'alkova Anna; Opletal Lubomir; Kunes Jiri; Novak Zdenek; Hrabinova Martina; Chlebek Jakub; Cegan Lukas; Cahlikova Lucie^*

来源：Natural Product Communications, 2015, 10(4): 577-580.

DOI：10.1177/1934578x1501000410

• Peumus boldus
• Monimiaceae
• Alkaloids
• Acetylcholinesterase
• Butyrylcholinesterase
• Prolyl oligopeptidase

Eleven isoquinoline alkaloids (1-11) were isolated from dried leaves of Peumus boldus Mol. by standard chromatographic methods. The chemical structures were elucidated by MS, and 1D and 2D NMR spectroscopic analysis, and by comparison with literature data. Compounds isolated in sufficient amount were evaluated for their acetylcholinesterase, and butyrylcholinesterase inhibition activity using Ellman's method. In the prolyl oligopeptidase assay, Z-Gly-Pro-p-nitroanilide was used as substrate. Promising butyrylcholinesterase inhibition activities were demonstrated by two bthizylisoquinoline alkaloids, reticuline (8) and N-methylcoclaurine (9), with IC50 values of 33.6 +/- 3.0 mu M and 15.0 +/- 1.4 mu M, respectively. Important prolyl oligopeptidase inhibition activities were shown by N-methyllaurotetanine (6) and sinoacutine (4) with IC50 values of 135.4 +/- 23.2 mu M and 143.1 +/- 25.4 mu M, respectively. Other tested compounds were considered inactive.

• 出版日期2015-4