Different cell death responses induced by eupomatenoid-5 in MCF-7 and 786-0 tumor cell lines

作者：Longato Giovanna Barbarini^*; Fiorito Giovanna Francisco; Vendramini Costa Debora Barbosa; de Oliveira Sousa Ilza Maria; Tinti Sirlene Valerio; Tasca Gois Ruiz Ana Lucia; Vitalino de Almeida Sinara Monica; Ribeiro Padilha Rafael Jose; Foglio Mary Ann; de Carvalho Joao Ernesto

来源：Toxicology in Vitro, 2015, 29(5): 1026-1033.

DOI：10.1016/j.tiv.2015.04.002

• Piper regnellii
• Eupomatenoid-5
• MCF-7
• 786-0
• Apoptosis
• Programmed necrosis

Natural products remain an important source of new drugs, including anticancer drugs. Recently, our group reported the anticancer activity of eupomatenoid-5 (eup-5), a neolignan isolated from Piper regnellii (Miq.) C. DC. var. regnellii leaves. In vitro studies demonstrated that MCF-7 (breast) and 786-0 (kidney) were among the cancer cell lines most sensitive to eup-5 treatment. The current results demonstrate that mitochondrial membrane depolarization and generation of reactive oxygen species are implicated in eup-5-mediated cytotoxic effects on these cancer cells lines. In MCF-7 cells, eup-5 led to phosphatidylserine externalization and caspase activation, whereas the same did not occur in 786-0 cells. Scanning electron microscopy revealed a reduction of microvilli density, as well as cell morphology alterations. Moreover, treated MCF-7 cells exhibited well-characterized apoptosis alterations, while treated 786-0 cells exhibited characteristics of programmed necroptosis process. These findings support the possibility that different mechanisms may be targeted by eup-5 in cell death response.

• 出版日期2015-8