Activation of Signal Transducer and Activator of Transcription 3 Signaling Attenuates Neurogenesis in a Rat Model of Intracerebral Hemorrhage

作者：Li, Hai-Tao; Zhou, Hua-Jun^*

来源：Turkish Neurosurgery, 2020, 30(6): 793-798.

DOI：10.5137/1019-5149.JTN.23665-18.1

• Intracerebral hemorrhage
• Neurogenesis
• Signal transducer and activator of transcription 3
• Rat

AIM: To investigate the effect of signal transducer and activator of transcription 3 (STAT3) signaling on neurogenesis following intracerebral hemorrhage (ICH). @@@ MATERIAL and METHODS: A rat model of ICH was induced via the stereotaxic injection of 100 mu L autologous blood into the right globus pallidus. AG490 (0.25 mg/kg) was injected into the lateral ventricle after ICH to block STAT3 signaling. Brains were perfused to identify proliferating cell nuclear antigen (PCNA)+/ doublecortin (DCX) + nuclei by immunohistochemistry. In addition, the distribution and expression of phosphorylated STAT3 [p-STAT3 (Tyr705)] were evaluated by immunohistochemistry and western blot, respectively. @@@ RESULTS: An enhanced level of p-STAT3 (Tyr705) was observed in neurons in the perihematomal region. An increase in DCX-positive cells and PCNA+/DCX+ nuclei was detected after ICH. Blocking STAT3 signaling with AG490 further increased the numbers of DCX-positive cells and PCNA+/DCX+ nuclei after ICH. @@@ CONCLUSION: These results suggest that activation of STAT3 signaling attenuates ICH-induced neurogenesis and may delay neural recovery following hemorrhagic cerebral injury.

• 出版日期2020
• 单位三峡大学