Nanoindentation of Pseudomonas aeruginosa bacterial biofilm using atomic force microscopy

作者：Baniasadi Mahmoud; Xu Zhe; Gandee Leah; Du Yingjie; Lu Hongbing; Zimmern Philippe; Minary Jolandan Majid^*

来源：Materials Research Express, 2014, 1(4): 045411.

DOI：10.1088/2053-1591/1/4/045411

• nanoindentation
• bacterial biofilm
• atomic force microscope
• nanomechanical properties
• pseudomonas aeruginosa

Bacterial biofilms are a source of many chronic infections. Biofilms and their inherent resistance to antibiotics are attributable to a range of health issues including affecting prosthetic implants, hospital-acquired infections, and wound infection. Mechanical properties of biofilm, in particular, at micro-and nanoscales, are governed by microstructures and porosity of the biofilm, which in turn may contribute to their inherent antibiotic resistance. We utilize atomic force microscopy (AFM)-based nanoindentation and finite element simulation to investigate the nanoscale mechanical properties of Pseudomonas aeruginosa bacterial biofilm. This biofilm was derived from human samples and represents a medically relevant model.

• 出版日期2014-12