A Robust and Poisson Validated Quantitative 5 ' Nuclease TaqMan (R) Real-Time PCR Assay Targeting fimA for the Rapid Detection of Salmonella spp. in Food

作者：Mann E; Hein I; Mester P; Stessl B; Rossmanith P; Wagner M; Dzieciol M^*

来源：Food Analytical Methods, 2013, 6(4): 991-995.

DOI：10.1007/s12161-012-9534-z

• fimA
• IAC
• qPCR
• Quantitative
• Salmonella

A newly designed TaqManA (R) probe and an internal amplification control were implemented in a conventional PCR system targeting the major fimbrial subunit encoding gene fimA. This assay has an inclusivity and exclusivity of 100 % (n = 126). The limit of detection (LOD) and the absolute quantification limit, both determined by advanced Poisson analyses, were three bacterial cell equivalents per quantitative real-time PCR reaction. The fimA assay achieved 100 % accuracy and performed very robustly, producing reproducible, reliable data for quantification of Salmonella spp., even at the LOD.

• 出版日期2013-8