High frequency of glucose-utilizing mutants in Shewanella oneidensis MR-1

作者：Howard Erinn C; Hamdan Leila J; Lizewski Stephen E; Ringeisen Bradley R^*

来源：FEMS Microbiology Letters, 2012, 327(1): 9-14.

DOI：10.1111/j.1574-6968.2011.02450.x

• GASP mutants
• Shewanella oneidensis MR-1
• glucose use
• mutator bacteria
• microbial fuel cells

Shewanella oneidensis MR-1 has conventionally been considered unable to use glucose as a carbon substrate for growth. The genome sequence of S. oneidensis MR-1 however suggests the ability to use glucose. Here, we demonstrate that during initial glucose exposure, S. oneidensis MR-1 quickly and frequently gains the ability to utilize glucose as a sole carbon source, in contrast to wild-type S. oneidensis, which cannot immediately use glucose as a sole carbon substrate. High-performance liquid chromatography and 14C glucose tracer studies confirm the disappearance in cultures and assimilation and respiration, respectively, of glucose. The relatively short time frame with which S. oneidensis MR-1 gained the ability to use glucose raises interesting ecological implications.

• 出版日期2012-2