AS04-adjuvanted virus-like particles containing multiple M2 extracellular domains of influenza virus confer improved protection

作者：Lee Yu Na; Kim Min Chul; Lee Young Tae; Hwang Hye Suk; Cho Min Kyoung; Lee Jong Seok; Ko Eun Ju; Kwon Young Man; Kang Sang Moo^*

来源：Vaccine, 2014, 32(35): 4578-4585.

DOI：10.1016/j.vaccine.2014.06.040

• Influenza virus
• M2e VLPs
• Adjuvants
• C57BL/6

The ectodomain of matrix protein 2 (M2e) of influenza virus is suggested to be a rational target for a universal influenza A vaccine. However, there are some concerns that M2e vaccines might not be highly effective in the general population with diverse genetic backgrounds. Here we examined the immuno-genicity and protective efficacy of the baculovirus-derived virus-like particles containing multiple M2e (M2eVLP) with AS04 adjuvant in a C57BL/6 mouse strain (H-2(b)). M2eVLP vaccine induced significant levels of M2e-specific IgG in C57BL/6 mice after vaccination. Furthermore, M2eVLP adjuvanted with AS04 was more effective than M2eVLP alone in conferring protection as well as in inducing recall humoral and T cell responses specific for M2e after lethal influenza virus challenge. A mechanistic study provides evidence that activation of dendritic cells by the toll-like receptor 4 agonist MPL in the AS04 adjuvant was associated with interferon-gamma producing CD4 T cell responses. Our results suggest that AS04 adjuvanted M2eVLP vaccines have the potential to improve cross-protection.

• 出版日期2014-7-31