NMR methods for measuring lateral diffusion in membranes

作者：Macdonald Peter M^*; Saleem Qasim; Lai Angel; Morales Hannah H

来源：Chemistry and Physics of Lipids, 2013, 166: 31-44.

DOI：10.1016/j.chemphyslip.2012.12.004

• Lateral diffusion
• Pulsed field gradient NMR
• Exchange NMR
• Vesicles
• Bicelles
• Macroscopically oriented membranes

Lateral diffusion is a fundamental property of biological membrane components, important for a host of biomembrane functions. Although long studied, novel aspects of the relationship between the structure of membrane components and their lateral diffusion properties continue to emerge. NMR-based lateral diffusion measurements are complicated by the spectral broadening arising from the slow anisotropic motions in membranes. Nevertheless, both pulsed field gradient (PFG) and exchange spectroscopy (EXSY) methods can be adapted to permit NMR measurements of lateral diffusion in membranes. These variously will be described in overview, highlighting advantages and limitations of each, but with particular emphasis on results from our laboratory using (1) H PFG NMR measurements in magnetically aligned bicelles and (31) P CODEX (Centreband-Only-Detection-of-Exchange) measurements in spherical phospholipid vesicles.

• 出版日期2013-1