科研之友
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摘要
In the present study we evaluated the apoptosis inducing activity of Luffa acutangula fruit extracts in leukemia model. Our results show that partially purified methanolic extract, F-3, dose dependently induced apoptosis in leukemia cell line HL-60. Initially apoptosis inducing activity of fraction F-3 was evaluated by observing morphological changes in HL-60, our results show that F-3 fraction treated HL-60 cells show characteristic morphology of apoptosis such as nuclear bebbling and budding of apoptosis bodies. In addition, F-3 fraction treated HL-60 cells have shown increased sub G0/G1 population and DNA fragment ladder as compared to control cells. To delineate the molecular mechanism F-3 treated cells were subjected to western blotting. Further more, FACS analysis show increased population in annexine positive in F-3 treated cells. Our results show that antiapoptotic protein Bcl2 expression was down regulated whereas, proapoptotic protein Bax was up regulated as compared to control cells. Apoptosis markers, cleaved caspase3 and PARP were dose dependently up regulated by F-3 treatment. Finally to test whether fraction F-3 induced apoptosis is mediated by extrinsic or intrinsic pathway, F-3 fraction treated HL-60 cells were analyzed for mitochondrial membrane depolarization and our results show that F-3 fraction significantly depolarizing the mitochondrial membrane in HL-60 cells indicating apoptosis induction by F-3 fraction is mediated may be by intrinsic pathway.
- 出版日期2010
