Direct and rapid electrochemical immunosensing system based on a conducting polymer

作者：Piro B; Zhang Q D; Rei**erg S; Noel V; Dang L A; Duc H T; Pham M C^*

来源：Talanta, 2010, 82(2): 608-612.

DOI：10.1016/j.talanta.2010.05.015

• Conjugated polymer
• Electrochemical immunosensor
• Reagentless detection
• Antibody-antigen affinity
• ELISA assays

A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen-antibody interaction model using ovalbumin-anti-ovalbumin. It was shown that the system specifically detects via electrochemical signal the antigen-antibody immune interaction in a reagentless context. Comparison to the conventional ELISA technique relevant to performance and sensitivity was presented.

• 出版日期2010-7-15