Nutritional regulation of actinomycin-D production by a new isolate of Streptomyces sindenensis using statistical methods

作者：Praveen Vandana; Tripathi Divya; Tripathi C K M^*; Biharil Vinod

来源：Indian Journal of Experimental Biology, 2008, 46(2): 138-144.

• actinomycin-D
• fermentation
• statistical medium optimization
• Streptomyces

Production of actinomycin-D, by an isolate, S. sindenensis, was optimized by statistical methods. Fructose peptone and NaNO3 were found to be critical for antibiotic production. In the second step, their concentrations were optimized with Central Composite Design and Response Surface Methodology. Fructose, peptone and NaNO3 at 2.55, 0.309 and 0.114 % respectively gave similar to 261 % higher yield (289 mg/l). Cultivation in fermentor at 600 rpm agitation and 1.5 vvm aeration with optimized medium gave 3.56 folds higher yield (365 mg/l) as compared to the yields in shake flasks using normal production medium (80 mg/l).

• 出版日期2008-2