Activities of enzymes that hydrolyze adenine nucleotides in platelets from patients with rheumatoid arthritis

作者：Becker Lara V; Rosa Cintia S; Souza Viviane do C G; Bagatini Margarete D; Casali Emerson A; Leal Claudio Alberto M; da Silva Joao Carlos N; Moretto Maria Beatriz; Pinheiro Francielle de V; Morsch Vera Maria; Schetinger Maria Rosa C; Leal Daniela B R^*

来源：Clinical Biochemistry, 2010, 43(13-14): 1096-1100.

DOI：10.1016/j.clinbiochem.2010.06.011

• Rheumatoid arthritis
• Platelets
• Adenine nucleotides
• Adenosine
• Atherosclerosis

Objectives: To investigate whether there are changes in the activity of the enzymes NTPDase, 5'-nucleotidase, E-NPP and ADA in platelets from patients with rheumatoid arthritis (RA).
Design and methods: Thirty-five RA patients diagnosed with RA through American College of Rheumatology criteria, as well as 35 healthy patients were selected. NTPDase, 5'-nucleotidase, E-NPP and ADA activities were verified in platelets isolated from these patients.
Results: The results demonstrate that an increase in NTPDase (approximately 100%), 5'-nucleotidase (170%), E-NPP (approximately 100%) and ADA (approximately 45%) activities occurred in RA patients when compared to the control group.
Conclusions: Ours results suggest an increase in the NTPDase, 5'-nucleotidase and E-NPP activities, which could be related to a compensatory organic response to excessive platelet aggregation which occurs during the inflammation. The increased ADA activity found in this work could lead to a decrease in the adenosine concentration in the circulation, which could explain the accelerated atherosclerosis found in patients with RA.

• 出版日期2010-9