Antioxidant and antiproliferative activity of Laurus nobilis L. (Lauraceae) leaves and seeds essential oils against K562 human chronic myelogenous leukaemia cells

作者：Saab Antoine M; Tundis Rosa^*; Loizzo Monica R; Lampronti Ilaria; Borgatti Monica; Gambari Roberto; Menichini Federica; Esseily Fadi; Menichini Francesco

来源：Natural Product Research, 2012, 26(18): 1741-1745.

DOI：10.1080/14786419.2011.608674

• Laurus nobilis
• antioxidant activity
• chronic myelogenous leukaemia
• antiproliferative activity
• synergism

The antioxidant and antiproliferative activities of the essential oils from Laurus nobilis leaves and seeds in relation to their composition were analysed. The most abundant components of the leaf essential oil were 1,8-cineole, 1-p-menthen-8-ethyl acetate, linalool and sabinene, while the seed oil was characterised by beta-ocimene, 1,8-cineole, alpha-pinene and beta-pinene as main constituents. Both seed and leaf essential oils exhibited a scavenging effect on the DPPH radical, with IC50 values of 66.1 and 53.5 mu g mL(-1), respectively. The leaf essential oil showed the strongest antioxidant activity in the beta-carotene/linoleic acid system, with an IC50 value of 35.6 mu g mL(-1) after 30 min of incubation. Both leaf and seed oils inhibited proliferation of the K562 tumour cell line with IC50 values of 95 and 75 mu gmL(-1), respectively. The L. nobilis leaf oil showed a percentage of erythroide differentiation of 15% at a concentration of 10 mu gmL(-1). A value of 12% was found for the seed essential oil at a concentration of 50 mu g mL(-1). When the oils were added to a suboptimal concentration of the commercial drug, cytosine arabinoside, a clear synergic effect was observed.

• 出版日期2012