A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene

作者：Moon, Heegyum; Cho, Sunghee; Loh, Tiing Jen; Zhou, Jianhua^*; Ghigna, Claudia; Biamonti, Giuseppe; Green, Michael R.; Zheng, Xuexiu; Shen, Haihong

来源：Oncology Reports, 2014, 31(1): 450-455.

DOI：10.3892/or.2013.2835

• Ron
• proto-oncogene
• cancer
• pre-mRNA splicing
• exon 11 inclusion
• cis-elements

Ron is a human receptor for the macrophage-stimulating protein (MSP). Exon 11 skipping of Ron pre-mRNA produces the Ron165 protein that has a deletion of a 49 amino acid region in the -chain extracellular domain. Ron165 is constitutively active even in the absence of its ligand. Through stepwise deletion analysis, we identified a 2-nt RNA enhancer, which is located 74 nt upstream from the 5 splice site of exon 11, for exon 11 inclusion. Through double-base and single-base substitution analysis of the 2-nt RNA, we demonstrated that the GA, CC, UG and AC dinucleotides on exon 11, in addition to the wild-type AG sequence, function as enhancers for exon 11 inclusion of the Ron pre-mRNA.

• 出版日期2014-1
• 单位南通大学