MULTIPLE LEADER SEQUENCES FOR MOUSE CATHEPSIN-B MESSENGER-RNA

作者：RHAISSI H^*; BECHET D; FERRARA M

来源：Biochimie, 1993, 75(10): 899-904.

DOI：10.1016/0300-9084(93)90046-U

• CATHEPSIN-B
• GENE STRUCTURE
• LEADER SEQUENCE

We previously described the gene structure of murine cathepsin B. Our results suggested that the 5'-untranslated region (leader) is interrupted by a large intron. The second exon (exon-2) contains the translation initiation site. To characterize the leader region, a rapid amplification of cDNA ends (RACE) procedure was developed. The PCR products were directly cloned and sequenced. Nucleotide sequence analyses revealed three different 5'-cDNA ends, suggesting the existence of three different leader regions. In addition to the leader (LA) previously characterized, we now describe two other 5'-untranslated regions, LB and LC. Leader LB is located 2.3 kb upstream exon-2, and leader LC corresponds to the 3'-end of the first intron and is thus contiguous to exon-2. Our results suggest for murine cathepsin B gene the presence of multiple promoters, and possibly the expression of multiple mRNAs differing in their leader region.

• 出版日期1993