High-Resolution N-6-Methyladenosine (m(6)A) Map Using Photo-Crosslinking-Assisted m(6)A Sequencing

作者：Chen, Kai; Lu, Zhike; Wang, Xiao; Fu, Ye; Luo, Guan-Zheng; Liu, Nian; Han, Dali; Dominissini, Dan; Dai, Qing; Pan, Tao; He, Chuan^*

来源：Angewandte Chemie - International Edition, 2015, 54(5): 1587-1590.

DOI：10.1002/anie.201410647

• N-6-methyladenosine
• photo-crosslinking
• RNA modification
• transcriptome sequencing

N-6-methyladenosine (m(6)A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles in mRNA metabolism. However, methods to precisely locate the m(6)A modification remain limited. We present here a photo-crosslinking-assisted m(6)A sequencing strategy (PA-m(6)A-seq) to more accurately define sites with m(6)A modification. Using this strategy, we obtained a high-resolution map of m(6)A in a human transcriptome. The map resembles the general distribution pattern observed previously, and reveals new m6A sites at base resolution. Our results provide insight into the relationship between the methylation regions and the binding sites of RNA-binding proteins.

• 出版日期2015-1-26