Nanoporous scaffold for DNA polymerase: pore-size optimisation of mesoporous silica for DNA amplification

作者:Matsuura Shun ichi*; Baba Tomoya; Chiba Manami; Tsunoda Tatsuo
来源:RSC Advances, 2014, 4(49): 25920-25923.
DOI:10.1039/c4ra02725f

摘要

Composites of thermostable DNA polymerase and mesoporous silicas with different pore diameters were used for DNA amplification. The enzyme was selectively immobilised on the mesopores, and DNA amplification activity was retained by regulating the pore size of the mesoporous silicas.

  • 出版日期2014