Depletion of neutrophils in a protective model of pulmonary cryptococcosis results in increased IL-17A production by gamma/delta T cells

作者：Wozniak Karen L; Kolls Jay K; Wormley Floyd L Jr^*

来源：BMC Immunology, 2012, 13: 65.

DOI：10.1186/1471-2172-13-65

• Gamma-delta T cells
• Cryptococcus neoformans
• IL-17A
• Pulmonary

Protective responses in mice immunized with an interferon-gamma producing strain of Cryptococcus neoformans, H99 gamma, are associated with IL-17A production by neutrophils. Neutrophil depletion in H99 gamma-immunized mice did not affect pulmonary fungal burden, indicating that neutrophils are not required for clearance. However, we observed an increase in IL-17A in the lungs of neutrophil-depleted H99 gamma infected mice, which corresponded to an increase in IL-17A(+) gamma delta(+) T cells. Moreover, we observed increased IL-17A(+)/CD3(+) cells and IL-17A(+)/gamma delta(+) cells, but decreased IL-17A(+)/Ly6G(+) neutrophils in the lungs of IL-17 receptor (R)A deficient mice compared to wild-type mice. Increased production of IL-17A in neutropenic mice coincided with increased IL-6 and CXCL1, but not Th17 inducing cytokines TGF-beta, IL-21 and IL-23. Concurrent depletion of neutrophils and gamma delta(+) T cells reduced IL-17A levels. Our results suggest that gamma delta(+) T cells mediate significant IL-17A production in neutropenic mice during the protective response to C. neoformans infection.

• 出版日期2012-12-7