A regenerable piezoelectric immunosensor on the basis of electropolymerized polypyrrole for highly selective detection of Staphylococcal Enterotoxin A in foodstuffs

作者：Karaseva Nadezhda^*; Ermolaeva Tatyana

来源：Microchimica Acta, 2015, 182(7-8): 1329-1335.

DOI：10.1007/s00604-015-1456-1

• Piezoelectric immunosensor
• Antibodies
• Immobilization
• Staphylococcal enterotoxin A
• Polypyrrole

Piezoelectric immunosensors have been developed for the determination of trace concentrations of Staphylococcal enterotoxin A (SEA) in foodstuff. Antibodies against SEA were covalently immobilized on an electropolymerized polypyrrole substrate on a gold electrode which warrants increased stability of the biolayer and sensitive detection. The calibration plot is linear in the 1-80 nga (TM) mLaEuro3/4(1) SEA concentration range, the limit of detection is 0.4 nga (TM) mLaEuro3/4(1), and the time of analysis is < 30 min. The sensor was successfully applied to quantify SEA in chicken meat and milk.

• 出版日期2015-6