Antibiotic Resistance Patterns and Detection of bla(DHA-1) in Salmonella Species Isolates from Chicken Farms in South Korea

作者：Rayamajhi Nabin; Jung Byeong Yeal; Bin Cha Seung; Shin Min Kyung; Kim Aeran; Kang Min Su; Lee Kang Mu; Yoo Han Sang^*

来源：Applied and Environmental Microbiology, 2010, 76(14): 4760-4764.

DOI：10.1128/AEM.02536-09

Fifteen nonrepetitive ampicillin-resistant Salmonella spp. were identified among 91 Salmonella sp. isolates during nationwide surveillance of Salmonella in waste from 131 chicken farms during 2006 and 2007. Additional phenotyping and genetic characterization of these 15 isolates by using indicator cephalosporins demonstrated that resistance to ampicillin and reduced susceptibility to cefoxitin in three isolates was caused by TEM-1 and DHA-1 beta-lactamases. Plasmid profiling and Southern blot analysis of these three DHA-1-positive Salmonella serovar Indiana isolates and previously reported unrelated clinical isolates of DHA-1-positive Salmonella serovar Montevideo, Klebsiella pneumoniae, and Escherichia coli from humans and swine indicated the involvement of the large-size plasmid. Restriction enzyme digestion of the plasmids from the transconjugants showed variable restriction patterns except for the two Salmonella serovar Indiana isolates identified in this study. To the best of our knowledge, this is the first report of the presence of the DHA-1 gene among Salmonella spp. of animal origin.

• 出版日期2010-7