Development of a Microsatellite-Based Method for the Differentiation of European Wild Boar (Sus scrofa scrofa) from Domestic Pig Breeds (Sus scrofa domestica) in Food

作者：Conyers Christine M^*; Allnutt Theodore R; Hird Heather J; Kaye Joy; Chisholm James

来源：Journal of Agricultural and Food Chemistry, 2012, 60(13): 3341-3347.

DOI：10.1021/jf205109b

• wild boar
• domestic pig
• SSR
• food authenticity
• Bayesian analysis of population structure

Twenty microsatellites (simple sequence repeats, SSR) were used to discriminate wild boar from domestic pig and to identify mixtures of the two. Reference groups of wild boar and pig samples were collected from the UK and Europe for genetic assignment tests. Bayesian Analysis of Populations software (BAPs) gave 100% correct assignment for blind wild boar and pig samples and correctly identified mixed samples. DNA was extracted from 12 commercial food samples (11 labeled as containing wild boar) including pates, salamis, and sausage, and good SSR profiles were obtained. Eleven samples were correctly assigned as pig, and two as mixed meats. One sample sold as wild boar meat was clearly assigned as pig. A further 10 blind samples of meat cuts were analyzed, eight wild boar and two pig, and all were correctly assigned.

• 出版日期2012-4-4