Ultrasensitive cDNA Detection of Dengue Virus RNA Using Electrochemical Nanoporous Membrane-Based Biosensor

作者：Rai Varun^*; Hapuarachchi Hapuarachchige C; Ng Lee Ching; Soh Siew Hwa; Leo Yee Sin; Toh Chee Seng

来源：PLos One, 2012, 7(8): e42346.

DOI：10.1371/journal.pone.0042346

A nanoporous alumina membrane-based ultrasensitive DNA biosensor is constructed using 5%26apos;-aminated DNA probes immobilized onto the alumina channel walls. Alumina nanoporous membrane-like structure is carved over platinum wire electrode of 76 mm diameter dimension by electrochemical anodization. The hybridization of complementary target DNA with probe DNA molecules attached inside the pores influences the pore size and ionic conductivity. The biosensor demonstrates linear range over 6 order of magnitude with ultrasensitive detection limit of 9.55x10(-12) M for the quantification of ss-31 mer DNA sequence. Its applicability is challenged against real time cDNA PCR sample of dengue virus serotype1 derived from asymmetric PCR. Excellent specificity down to one nucleotide mismatch in target DNA sample of DENV3 is also demonstrated.

• 出版日期2012-8-23
• 单位南阳理工学院