A bio-barcode assay for on-chip attomolar-sensitivity protein detection

作者：Goluch Edgar D; Nam Jwa Min; Georganopoulou Dimitra G; Chiesl Thomas N; Shaikh Kashan A; Ryu Kee S; Barron Annelise E; Mirkin Chad A; Liu Chang^*

来源：Lab on a Chip, 2006, 6(10): 1293-1299.

DOI：10.1039/b606294f

Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles-a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using I mu L total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.

• 出版日期2006-10
• 单位西北大学