Establishment of a Clinic-based Biorepository

作者：Belden Sarah E; Uppalapati Chandana K; Pascual Agnes S; Montgomery McKale R; Leyva Kathryn J; Hull Elizabeth E^*; Averitte Richard L

来源：Jove-Journal of Visualized Experiments, 2017, (123): e55583.

DOI：10.3791/55583

• Medicine
• Issue 123
• Clinic-based biorepository
• skin cancer
• Mohs surgery
• squamous cell carcinoma
• tissue processing
• tissue bank

The incidence of skin cancer (e.g., squamous cell carcinoma, basal cell carcinoma, and melanoma) has been increasing over the past several years. It is expected that there will be a parallel demand for cutaneous tumor samples for biomedical research studies. Tissue availability, however, is limited due the cost of establishing a biorepository and the lack of protocols available for obtaining clinical samples that do not interfere with clinical operations. A protocol was established to collect and process cutaneous tumor and associated blood and saliva samples that has minimal impact on routine clinical procedures on the date of a Mohs surgery. Tumor samples are collected and processed from patients undergoing their first layer of Mohs surgery for biopsy-proven cutaneous malignancies by the Mohs histotechnologist. Adjacent normal tissue is collected at the time of surgical closure. Additional samples that may be collected are whole-blood and buccal swabs. By utilizing tissue samples that are normally discarded, a biorepository was generated that offers several key advantages by being based in the clinic versus the laboratory setting. These include a wide range of collected samples; access to de-identified patient records, including pathology reports; and, for the typical donor, access to additional samples during follow-up visits.

• 出版日期2017-5