Testing of microorganisms for omega-transaminase activity

作者：Clay Donna; Koszelewski Dominik; Grischek Barbara; Gross Johannes; Lavandera Ivan; Kroutil Wolfgang^*

来源：Tetrahedron: Asymmetry , 2010, 21(16): 2005-2009.

DOI：10.1016/j.tetasy.2010.07.009

Various bacterial cells were tested to identify omega-transaminase activity. For this purpose, the kinetic resolution of a rac-amine was chosen as an assay reaction transforming, in the ideal case, one enantiomer into the corresponding ketone and leaving the other enantiomer untouched. Sodium pyruvate was employed as an amino acceptor. To test also for the amination of the prochiral ketone various amino donors were investigated. Alanine proved to be the most suitable amino donor especially when coupled with a pyruvate decarboxylase to shift the reaction equilibrium; however, much lower conversions were achieved compared to the kinetic resolution. Janibacter terrae DSM 13953 was identified as the most suitable microorganism to possess w-transaminase activity.

• 出版日期2010-8-23