The unique and immunoglobulin-like regions of surrogate light chain component lambda 5 differentially interrogate immunoglobulin heavy-chain structure

作者：Smith Brendan P; Roman Christopher A J^*

来源：Molecular Immunology, 2010, 47(6): 1195-1206.

DOI：10.1016/j.molimm.2010.01.002

• B cells
• Antibodies
• Cell differentiation
• Repertoire development

PreBCR signaling is critical for B cell development and normally depends on the association of a nascent, component Ig H chain with the surrogate L chain (SLC), which helps ensure that only B cells that synthesize structurally sound antibody can develop. How the invariant and lambda-like SLC vets billions of unique V(H) domains for compatibility with polymorphic kappa and lambda L chains is unclear, because the SLC is composed of not only the Ig domains of VpreB and lambda 5, but also the unique regions (URs) that reside at what would be the L chain CDR3. We evaluated the contribution of the Ig and UR domains of lambda 5 to H chain screening by evaluating the preBCR-forming capability of lambda 5 mutants with a diverse panel of H chains. Using transformed mouse B cells, we demonstrate that the Ig domain of lambda 5 was sufficient and its UR dispensable for the rejection of V(H)Q52 and V(H)10 SLC-incompatible H chains. In contrast, the lambda 5 UR was necessary to discriminate between SLC-incompatible and -compatible V(H)81XH chains. Substituting the Ig domains of lambda 5 with equivalent K sequences impaired the SLC's ability to escort all H chains to the surface. Two SLC-incompatible H chains were able to form surface BCRs with two kappa L chains, indicating that the SLC's ability to predict the L chain compatibility of a H chain is not absolute. In sum, lambda 5 differentially relies on the lambda-like Ig and UR to probe H chain structure to best accommodate diversity among H chains.

• 出版日期2010-3