Paternity determination of captive bottlenose dolphins (Tursiops truncatus) using microsatellite DNA analysis

作者:Sumiyama Daisuke; Kitamura Shouichi; Terasawa Fumio; Hori Yukiko; Murata Koichi*; Kulski Jerzy K; Inoko Hidetoshi
来源:Journal of Veterinary Medical Science, 2008, 70(7): 711-713.
DOI:10.1292/jvms.70.711

摘要

We applied previously published PCR primer pairs to amplify alleles at three polymorphic microsatellite loci to determine the genetic relationship of 6 bottlenose dolphins (Tursiops truncatus) that were living together in a Japanese aquarium. The three microsatellite loci were sufficient to determine the haplotype relationships of the six dolphins, which represented three different generations. It was confirmed that this genotyping method is simple and economical for assessing, establishing and maintaining genetic diversity in captive populations and will become a very effective technique for ex situ conservation in aquariums and zoos.

  • 出版日期2008-7
  • 单位上海生物信息技术研究中心

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