科研之友
微信
新浪微博
Facebook
分享链接
摘要
C(0)t-1 DNA, enriched for repetitive DNA sequences, has been proved to be valuable in the studies of plant species differentiation and genome evolution. A new protocol to steadily obtain the aimed range of DNA fragments has been developed by shearing the genomic DNA with the digest system containing DNase I and DNA polymerase I. Comparison through fluorescence in situ hybridization (FISH) - Oryza sativa L with labeled probe of C(0)t-1 DNA isolated by the autoclave equipment and the new procedure was carried out. FISH result shows that the new procedure can be a feasible method to steadily isolate high-quality C(0)t-1 DNA.
