Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification

作者：Hong, Cheng-Yi; Chen, Xian; Li, Juan; Chen, Jing-Hua^*; Chen, Guonan; Yang, Huang-Hao

来源：Chemical communications, 2014, 50(25): 3292-3295.

DOI：10.1039/c3cc48996e

• RECOGNITION

We have developed a simple method for direct detection of circulating microRNAs in serum by using the p19 protein-functionalized magnetic beads (PFMBs) for specific enrichment and rolling circle amplification (RCA). The detection limit for microRNAs is 1 fM. Therefore, the proposed method has the potential of being used in the analysis of circulating microRNAs and clinical diagnosis.

• 出版日期2014
• 单位福建医科大学; 福州大学