ISOLATION AND PURIFICATION OF 20-HYDROXYECDYSONE FROM RADIX SERRATULAE CHINENSIS BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

作者：Huang, Jieyun; Xu, Xinjun^*; Xie, Zhiyong; Xie, Zhisheng; Yang, Mei; Yang, Depo

来源：Journal of Liquid Chromatography & Related Technologies, 2014, 37(13): 1909-1916.

DOI：10.1080/10826076.2013.825850

• HPLC
• Radix Serratulae Chinensis
• isolation
• purification
• HSCCC
• 20-Hydroxyecdysone

The bioactive compound 20-Hydroxyecdysone was successfully isolated and purified from Radix Serratulae Chinensis by high-speed counter-current chromatography with a solvent system of ethyl acetate-n-butanol-water (4:1:5, v/v/v) in one step. From 230mg of the n-butanol extract of Radix Serratulae Chinensis, 16.7mg of 20-Hydroxyecdysone was obtained with purity of 99.3%, as determined by high performance liquid Chromatography (HPLC). The structure was identified by mass spectrophotometry (MS), ultraviolet spectrophotometry (UV), and nuclear magnetic resonance spectrophotometry (NMR) analysis. In this study, a rapid method for isolation and purification of the major compound from Radix Serratulae Chinensis extract was established.

• 出版日期2014-8-9
• 单位中山大学