Determination of ochratoxin A in wine using liquid-phase microextraction combined with liquid chromatography with fluorescence detection

作者：Gonzalez Penas E^*; Leache C; Viscarret M; de Obanos AP; Araguas C; de Cerain AL

来源：Journal of Chromatography A, 2004, 1025(2): 163-168.

DOI：10.1016/j.chroma.2003.10.113

• wine
• liquid-phase microextraction
• mycotoxins
• ochratoxin A

A liquid-liquid microextraction technique (LPME) has been applied to the extraction of ochratoxin A (OTA) from wine prior to its quantification by HPLC-fluorescence detection. OTA was extracted from wine, through l-octanol immobilized in the pores of a porous hollow fiber, and introduced into l-octanol inside the fiber. Recovery was 77%. The method was adequate for quantification of OTA in wine at levels within the range 0.25-10 ng/ml with a LOD of 0.2 ng/ml, and can be a simple and inexpensive alternative to the use of innumoaffinity columns in order to quantify OTA levels in wine.

• 出版日期2004-2-6