A reversible surface functionalized nanowire transistor to study protein-protein interactions

作者：Lin Shu Ping; Pan Chien Yuan; Tseng Kun Chang; Lin Ming Chou; Chen Chii Dong; Tsai Chia Chang; Yu Shu Han; Sun Ying Chieh; Lin Tsung Wu; Chen Yit Tsong^*

来源：Nano Today, 2009, 4(3): 235-243.

DOI：10.1016/j.nantod.2009.04.005

• Nanowire
• Field-effect transistor
• Reversible surface functionalization
• Protein-protein interaction

Taking advantage of the reversible association-dissociation between glutathione (GSH) and glutathione S-transferase (GST), we have developed a novel method by surface-functionalizing GSH on silicon nanowire field-effect transistors (SiNW-FET) and then anchoring a particular GST-fused protein for the studies of protein-protein interactions. The reversible surface functionalization method has been proven by analytical techniques, fluorescence imaging, atomic force microscopic imaging, and electrical measurements. The reversible GSH-GST functionality on the SiNW-FET has made this sensorial device consecutively reusable and calibratable, allowing for quantitative analysis. In this work, we have demonstrated that the biologically modified SiNW-FET can be used as a screening sensor for the future studies of biomolecular associations, such as protein-protein interactions, protein-DNA interactions, protein-carbohydrate interactions, etc.

• 出版日期2009-6
• 单位中国科学院; 中国科学院电工研究所