科研之友
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摘要
We examined the effect of myricetin on cell dysfunction in cytokine-induced pancreatic beta cells and assessed whether Wnt signal pathway was the target of myricetin. RIN-m5f beta cells were exposed to a combination of tumor necrosis factor-alpha, interleukin-1 beta, and interferon-gamma, with or without myricetin pretreatment for 48 h. The cell viability, basal and glucose-stimulated insulin secretion and Wnt-signaling proteins were evaluated with methyl thiazolyl tetrazolium assay, radio immunoassay and Western blotting, respectively. The 48 h multiple-cytokine treatment decreased cell viability and glucose-stimulated insulin secretion, while increasing basal insulin secretion. Western blot analysis showed that Wnt-signaling proteins were decreased in cytokine-treated RIN-m5f cells. However, myricetin pretreatment protected against cytokine-induced cell death. In addition, myricetin (20 mu mol/L) obviously decreased basal insulin secretion and increased glucose-stimulated insulin secretion in cytokine-treated RIN-m5f cells. Western blot analysis showed that Wnt-signaling proteins were increased after myricetin pretreatment. Therefore, myricetin might attenuate cell dysfunction in cytokine-induced RIN-m5F cells via the Wnt signal pathway, and the Wnt signal pathway might be used as a new target for protecting pancreatic beta cells against cytokine-induced cell dysfunction and death.
- 出版日期2013-1
- 单位北京大学
