Application of magnetic hydroxyapatite nanoparticles for solid phase extraction of plasmid DNA

作者：Shan, Zhi^*; Li, Xianghai; Gao, Yaying; Wang, Xianxiang; Li, Chenglei; Wu, Qi

来源：Analytical Biochemistry, 2012, 425(2): 125-127.

DOI：10.1016/j.ab.2012.03.013

• Magnetic hydroxyapatite nanoparticles
• pDNA
• Purification

We developed a facile method for plasmid DNA (pDNA) extraction from crude Escherichia coli lysate using magnetic hydroxyapatite nanoparticles (MHapNPs) in the presence of polyethylene glycol (PEG)/NaCl. DNA condensation induced by PEG/NaCl is a prerequisite for achieving pronounced DNA recovery. The quality and quantity of MHapNP-purified pDNA under optimal binding buffer conditions (0.5 volume of 20% PEG 8000/2 M NaCl) were comparable to those obtained using organic solvents or commercial kits. This MHapNP technique is rapid, simple, cost-effective, and environmentally friendly and has the potential to extract DNA from other cell lysates.

• 出版日期2012-6-15
• 单位四川农业大学