Antibacterial activity of silver nanoparticles stabilized on tannin-grafted collagen fiber

作者：He, Li; Gao, Si-ying; Wu, Hao; Liao, Xue-pin^*; He, Qiang; Shi, Bi

来源：Materials Science & Engineering C-Materials for Biological Applications, 2012, 32(5): 1050-1056.

DOI：10.1016/j.msec.2011.07.024

• Collagen fiber
• Bayberry tannin
• Silver nanoparticles
• Antibacterial activity

Bayberry tannin (BT), a typical plant polyphenol, was grafted on collagen fiber (CF) in different mass ratios. Subsequently, the BT-grafted CF (BT-CF) was used as carrier and stabilizer to prepare BT-CF stabilized silver nanoparticles (BT-CF-AgNPs). Scanning Electron Microscopy image of BT-CF-AgNPs showed that the BT-CF-AgNPs was in ordered fibrous state. X-ray Diffraction patterns and Transmission Electron Microscopy images offered evidence that the Ag nanoparticles were well dispersed on BT-CF. Fourier Transform-Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopy (XPS) investigations revealed that the Ag NPs were stabilized by the phenolic hydroxyls and quinones of BT on CF through electron donation/acception interaction. Antibacterial experiments demonstrated that BT-CF-AgNPs exhibited high antibacterial activity. When cell suspensions of Escherichia coli and Staphylococcus aureus (10(4)-10(5) cfu/mL) were contacted with BT0.19-CF-AgNPs (mass ratio of BT to CF = 0.19, conc. of Ag = 8 mu g/mL) at 310 K under constant shaking, the number of cells went down to zero within 2 h. In addition, the minimal inhibitory concentration of BT0.19-CF-AgNPs against Escherichia coli, Staphylococcus aureus, Penicillium glaucum and Saccharomyces cerevisiae was 2 mu g/mL, 4 mu g/mL, 6 mu g/mL and 12 mu g/mL Ag, respectively. During recycling use, the antibacterial activity of BT0.19-CF-AgNPs against Escherichia coli can last for 5 cycles. These facts suggest that BT-CF-AgNPs can be used as a new and effective antibacterial agent.

• 出版日期2012-7-1
• 单位四川大学