Acetylsalicylic acid-triggered 15-HETE generation by peripheral leukocytes for identifying ASA sensitivity

作者：Korosec Peter^*; Tisler Ursa; Bajrovic Nissera; Silar Mira; Mrhar Ales; Kosnik Mitja

来源：Respiratory Medicine, 2011, 105: S81-S83.

DOI：10.1016/S0954-6111(11)70017-8

• Acetylsalicylic acid hypersensitivity
• 15-HETE
• Leukocytes

Background: Exposure to acetylsalicylic acid (ASA) may exacerbate respiratory or skin diseases or induce anaphylactoid reactions in apparently healthy individuals. We wanted to evaluate the clinical and diagnostic utility of measuring ASA-induced 15-hydroxyeicosatetraenoic acid (15-HETE) generation.
Methods: We performed a prospective single-blind study with 26 subjects undergoing clinical evaluation and/or ASA provocation testing. We also included 12 control subjects. Peripheral, blood leukocytes were incubated with 500 mu M ASA and 15-HETE release was measured by competitive ELISA.
Results: We found that 18 subjects were ASA-tolerant and 8 were ASA-intolerant. The mean increase in 15-HETE in intolerant subjects was 34% and this was comparable to the mean increase of 30% observed in ASA-tolerant subjects. A similar mean increase was also observed in control subjects. The ROC calculation showed that the optimal diagnostic threshold would be an increase of greater than 33%. However, the sensitivity of this increase was only 63% and the specificity was 50%.
Conclusions: Our data suggest that further studies are needed before the ASA-induced 15-HETE test can be used in clinical practice.

• 出版日期2011-10