ANTAGONISM OF Trichoderma SPP. AND Bacillus subtilis (UFV3918) TO Fusarium sambucinum IN Pinus elliottii ENGELM

作者：Maciel Caciara Gonzatto^*; Walker Clair; Briao Muniz Marlove Fatima; Araujo Maristela Machado

来源：Revista Arvore, 2014, 38(3): 505-512.

• Biocontrol
• Microbiolization
• Growth promotion

Pinus elliottii is an important species in the forest sector, which has restrictions such as seed sanitary quality, especially the association of Fusarium spp., responsible for seedling losses in the nursery. This study evaluated antagonist in vitro and in vivo action of Trichoderma spp. and Bacillus subtilis (UFV3918) in controlling Fusarium sambucinum, responsible for damage in Pinus elliottii seedlings. The in vitro assay considered the inhibition of mycelial growth (paired comparison of cultures) after incubation at 25 +/- 2 degrees C and photoperiod of 12 hours. For in vivo tests (performed in nursery conditions) seeds were initially inoculated with the pathogen and, subsequently, microbiolized with antagonistic agents, before sowing. The techniques of contact with biocontroller on PDA for 48 hours and seed coating, as forms of microbiolization, were used. Both Trichoderma spp. and Bacillus subtilis (UFV3918) were efficient on the in vitro control of F. sambucinum; however, Bacillus subtilis (UFV3918) was more effective against the pathogen on the in vivo biocontrol test, reducing seedlings losses caused by the pathogen and increasing values of length, as well as fresh and dry matter of the seedling.

• 出版日期2014-6