AKT2 expression changes impact on invasive ability of breast cancer cells

作者：Tan, Xiaolang; Guo, Lei^*; Luo, Shayang; Wang, Weiming; Zhang, Kejing; Chen, Feiyu

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(4): 4560-4565.

• AKT2
• breast cancer cell
• transwell assay
• cell transfection

AKT2 plays an important role in PI3K signaling pathway and directly relates to cell growth. Study showed that AKT2 was associated with tumor occurrence, growth, and invasion. This research aimed to detect AKT2 expression impact on breast cancer cell invasive ability, to provide support for clinical prognosis. AKE2 overexpression vector was constructed using pEGFP-N1 as the carrier and transfected to breast cancer cell line by liposome. RTPCR and Western blot were applied to test AKT2 expression at 12 h, 16 h, and 24 h after transfection, respectively. Transwell assay was performed to determine cell invasion. AKT2 overexpression vector pEGFP-AKT2 and inhibitory vector AKT2-sh-RNA were successfully constructed. After pEGFP-AKT2 transfection, AKT2 expression increased significantly, and cell invasive ability also enhanced. AKT2-sh-RNA transfection declined AKT2 level and reduced breast cancer cell invasive ability. AKT2 expression changes had certain relationship with breast cancer cell invasive ability. AKT2 overexpression may affect breast cancer prognosis.

• 出版日期2016
• 单位长沙市中心医院; 中南大学