科研之友
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摘要
A selective two phase liquid culture system for erythroid progenitors was developed to isolate and analyze mRNAs encoding erythrocyte-specific blood group antigens. The feasibility of the technique was verified by isolation and identification of mRNA for Rh polypeptide using polymerase chain reaction accompanied with the subcloning and sequencing methods. Two kinds of Rh polypeptide-specific cDNA sequences were demonstrated. It was revealed that each individual has one or both of them. This approach based on the culture system is widely applicable to studies to detect gene expression of any proteins of erythroid blood group antigens at the RNA level.
- 出版日期1992-4
