Nano-scale liquid chromatography coupled to tandem mass spectrometry using the multiple reaction monitoring mode based quantitative platform for analyzing multiple enzymes associated with central metabolic pathways of Saccharomyces cerevisiae using ultra fast mass spectrometry

作者：Matsuda Fumio^*; Ogura Tairo; Tomita Atsumi; Hirano Ichiro; Shimizu Hiroshi

来源：Journal of Bioscience and Bioengineering, 2015, 119(1): 117-120.

DOI：10.1016/j.jbiosc.2014.06.010

• Quantitative proteomics
• Ultra fast mass spectrometry
• Central carbon metabolism
• Saccharomyces cerevisiae
• Enzyme expression

A widely targeted quantitative proteome analysis of Saccharomyces cerevisiae enzymes was performed employing an ultra fast mass spectrometry (UFMS). Nano-liquid chromatography-UFMS analysis of trypsin digested peptide samples derived from yeast strains successfully determined relative abundances of 303 peptides of 137 proteins.

• 出版日期2015-1
• 单位RIKEN