Anti-miR-148a regulates platelet Fc gamma RIIA signaling and decreases thrombosis in vivo in mice

作者：Zhou Yuhang; Abraham Shaji; Andre Pierrette; Edelstein Leonard C; Shaw Chad A; Dangelmaier Carol A; Tsygankov Alexander Y; Kunapuli Satya P; Bray Paul F; McKenzie Steven E^*

来源：Blood, 2015, 126(26): 2871-2881.

DOI：10.1182/blood-2015-02-631135

Fc receptor for IgG IIA (Fc gamma RIIA)-mediated platelet activation is essential in heparin-induced thrombocytopenia (HIT) and other immune-mediated thrombocytopenia and thrombosis disorders. There is considerable interindividual variation in platelet FcgRIIA activation, the reasons for which remain unclear. We hypothesized that genetic variations between FcgRIIA hyper- and hyporesponders regulate Fc gamma RIIA-mediated platelet reactivity and influence HIT susceptibility. Using unbiased genome-wide expression profiling, we observed that human hyporesponders to FcgRIIA activation showed higher platelet T-cell ubiquitin ligand-2 (TULA-2) mRNA expression than hyperresponders. Silent interfering RNA-mediated knockdown of TULA-2 resulted in hyperphosphorylation of spleen tyrosine kinase following FcgRIIA activation in HEL cells. Significantly, we found miR-148a-3p targeted and inhibited both human and mouse TULA-2 mRNA. Inhibition of miR-148a in Fc gamma RIIA transgenic mice upregulated the TULA-2 level and reduced Fc gamma RIIA- and glycoprotein VI-mediated platelet alpha(IIb)beta(3) activation and calcium mobilization. Anti-miR-148a also reduced thrombus formation following intravascular platelet activation via Fc gamma RIIA. These results show that TULA-2 is a target of miR-148a-3p, and TULA-2 serves as a negative regulator of Fc gamma RIIA-mediated platelet activation. This is also the first study to show the effects of in vivo miRNA inhibition on platelet reactivity. Our work suggests that modulating miR-148a expression is a potential therapeutic approach for thrombosis.

• 出版日期2015-12-24