GRK5-Knockout Mice Generated by TALEN-Mediated Gene Targeting

作者：Nanjidsuren Tsevelmaa; Park Chae Won; Sim Bo Woong; Kim Sun Uk; Chang Kyu Tae; Kang Myung Hwa; Min Kwan Sik^*

来源：Animal Biotechnology, 2016, 27(4): 223-230.

DOI：10.1080/10495398.2016.1176032

• Gene targeting
• GRK-5
• Knockout
• TALEN

Transcription activator-like effector nucleases (TALENs) are a new type of engineered nuclease that is very effective for directed gene disruption in any genome sequence. We investigated the generation of mice with genetic knockout (KO) of the G protein-coupled receptor kinase (GRK) 5 gene by microinjection of TALEN mRNA. TALEN vectors were designed to target exons 1, 3, and 5 of the mouse GRK5 gene. Flow cytometry showed that the activity of the TALEN mRNAs targeted to exons 1, 3, and 5 was 8.7%, 9.7%, and 12.7%, respectively. The TALEN mRNA for exon 5 was injected into the cytoplasm of 180 one-cell embryos. Of the 53 newborns, three (5.6%) were mutant founders (F-0) with mutations. Two clones from F(0)28 showed a 45-bp deletion and F(0)39 showed the same biallelic non-frame-shifting 3-bp deletions. Three clones from F(0)41 were shown to possess a combination of frame-shifting 2-bp deletions. All of the mutations were transmitted through the germline but not to all progenies (37.5%, 37.5%, and 57.1% for the F(0)28, F(0)39, and F(0)41 lines, respectively). The homozygote GRK5-KO mice for 28 and 41 lines created on F3 progenies and the homozygous genotype was confirmed by PCR, T7E1 assay and sequencing.

• 出版日期2016