A laser-based technology for fabricating a soda-lime glass based microfluidic device for circulating tumour cell capture

作者：Nieto Daniel^*; Couceiro Ramiro; Aymerich Maria; Lopez Lopez Rafael; Abal Miguel; Teresa Flores Arias Maria

来源：Colloids and Surfaces B: Biointerfaces , 2015, 134: 363-369.

DOI：10.1016/j.colsurfb.2015.07.007

• Laser ablation
• Glass materials
• Microfluidics devices
• Circulating tumour cells

We developed a laser-based technique for fabricating microfluidic microchips on soda-lime glass substrates. The proposed methodology combines a laser direct writing, as a manufacturing tool for the fabrication of the microfluidics structures, followed by a post-thermal treatment with a CO2 laser. This treatment will allow reshaping and improving the morphological (roughness) and optical qualities (transparency) of the generated microfluidics structures. The use of lasers commonly implemented for material processing makes this technique highly competitive when compared with other glass microstructuring approaches. The manufactured chips were tested with tumour cells (Hec 1A) after being functionalized with an epithelial cell adhesion molecule (EpCAM) antibody coating. Cells were successfully arrested on the pillars after being flown through the device giving our technology a translational application in the field of cancer research.

• 出版日期2015-10-1