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摘要
In the biosensor construction, 3-mercaptopropionic acid (3-MPA) and 6-aminocaproic acid (6-ACA) were used for forming self-assembled monolayer (SAM) on a gold disc electrode and pyruvate oxidase was immobilized on the modified electrode surface by using glutaraldehyde. Biosensor response is linearly related to pyruvate concentration at 2.5-50 mu M, detection limit is 1.87 mu M and response time of the biosensor is 6 s for differential pulse voltammograms. From the repeatability studies (n = 6) for 30.0 mu M pyruvate revealed that the average value ((x) over bar), standard deviation (S. D) and coefficient of variation (CV %) were calculated to be 31.02 mu M, +/- 0.1914 mu M and 0.62%, respectively.
- 出版日期2014-12
