Mitochondrial haplogroups and expression studies of commonly used human cell lines

作者：Siegismund Christine Sabine^*; Schaefer Ingo; Seibel Peter; Kuehl Uwe; Schultheiss Heinz Peter; Lassner Dirk

来源：Mitochondrion, 2016, 30: 236-247.

DOI：10.1016/j.mito.2016.08.012

• Cell lines
• Multiplex fragment length analysis
• Mitochondrial haplogroup
• Mitochondria
• mtDNA
• Gene expression analysis

We developed a multiplex fragment length analysis (MFLA) for clearly assigning mitochondrial haplogroups mostly endemic in Europe for future cardiac diagnostics. As a technical proof, 23 commonly used human cell lines were haplotyped as reference standards. The functional analysis on mtDNA copies per cell revealed no correlation to haplogroups but a relatively high rate of mitochondria per cell and at the same time a very low expression of all mitochondrial and some nuclear encoded mitochondrial related genes. Established MFLA is an easy to handle method for analysing European mitochondrial haplogroups to perform epidemic studies and elucidate correlations to distinct diseases.

• 出版日期2016-9