Embryonic developmental toxicity of selenite in zebrafish (Danio rerio) and prevention with folic acid

作者：Ma, Yan; Wu, Min; Li, Dan; Li, Xiao-qiao; Li, Peng; Zhao, Jun; Luo, Min-na; Guo, Chang-long; Gao, Xiao-bo; Lu, Cai-ling^*; Ma, Xu

来源：Food and Chemical Toxicology, 2012, 50(8): 2854-2863.

DOI：10.1016/j.fct.2012.04.037

• Selenite embryotoxicity
• Zebrafish
• Heart development
• Neural development
• Folic acid
• DNA methylation

Selenium (Se) is an essential micronutrient, but also a potential toxin, which may be absorbed in excess. Relatively little is known about selenium embryotoxicity in zebrafish. In this study, we evaluated the effect of selenite exposure in zebrafish embryos. Selenite treatment decreased survival and resulted in abnormal development in a dose- and time-dependent manner. We observed irregular growth of neurons in selenite treated embryos, characterized by the absence of neurons in the brain, trunk and tail. Selenite exposure also induced defects in heart function, such as bradycardia and cardiac dysplasia with irregular and smaller chamber shape. In addition, selenite exposure caused ectopic cell proliferation, apoptosis, and a change in the pattern of DNA methylation. Our results suggested that supplementation with folic acid (FA) ameliorated the cardiac and neural defects in selenite-treated embryos. In conclusion, we demonstrated that selenite exposure caused cardiac and neural defects in zebrafish embryos and that folic acid protected against this embryotoxicity. It will give insight into the risk assessment and prevention of Se-mediated embryotoxicity.

• 出版日期2012-8
• 单位国家卫生计生委科学技术研究所; 中国医学科学院北京协和医院